Diesmal ist das Thema in der Festkörperphysik zu finden: es geht um NMR-Spektroskopie an DOPC-Membranen, unter der Anwendung der LG-HETCOR-LG-Methode.
Abstract:
Direct observation of J-couplings remains a challenge in high-resolution solid-state NMR. In some cases, it is possible to use Lee–Goldburg (LG) homonuclear decoupling during rare spin observation in MAS NMR correlation spectroscopy of lipid membranes to obtain J-resolved spectra in the direct dimension. In one simple implementation, a wide line separation-type
13C-1H HETCOR can provide high-resolution 1H/13C spectra, which are J-resolved in both dimensions. Coupling constants, 1JCH, obtained from 1H doublets, can be compared with scaled 1JθCH obtained from the 13C multiplets to assess the LG-values efficiency and scaling factor. The use of homonuclear decoupling during proton evolution, LG-HETCOR-LG, can provide J-values, at least in the rare spin dimension, and allows measurements in less mobile membrane environments. The LG-decoupled spectroscopic approach is demonstrated on pure dioleoylphosphatidylcholine (DOPC) membranes and used to investigate lipid mixtures of DOPC/cholesterol and DOPC/cholesterol/sphingomyelin.
Die Autoren sind Mitarbeiter der Universität von Nottingham in Großbritannien.
Erschienen ist der Artikel im Magazin Magnetic Resonance in Chemistry, Volume 48, Teil 12, Seiten 925–934. Online zu finden ist er außerdem unter DOI 10.1002/mrc.2690.
